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Based on the premise that the physiological range of oxygen in tissues is between 1- 8%, and pathologies from cancer to diabetes are characterised by much lower oxygen levels, researchers worldwide are cultivating their cell cultures in the Hypoxystation by Don Whitley Scientific. The Hypoxystation provides physiologically relevant conditions for cell culture and manipulation to ensure authentic behaviour of cells. User-defined parameters for temperature, CO2, O2 and humidity, plus the workstation format, where cells reside throughout the entire duration of the assays, minimise the extra-physiologic oxygen shock that is known to negatively impact cell metabolism and growth.
Numerous recent publications by our Hypoxystation users demonstrate that cell culture conditions which mimic physioxia, are essential in avoiding the significantly impaired growth rates, reduced lifespan, and altered molecular behaviour encountered in cells cultivated at ambient conditions. Oxygen levels in tissues are in constant flux; they change in response to functional status and blood delivery in the organs, and this too can be re-created in the Hypoxystation through programmed oxygen profiling.
Recent research using the Hypoxystation to investigate hypoxia inducible factors and the array of signalling pathways that regulate angiogenesis, metabolism, redox homeostasis, inflammation, and cell death, and the many other processes which enable the cellular and organismal response to hypoxia, “highlights the importance of oxygen as a cell culture parameter when making physiological inferences” (Timpano and Uniacke, 2016).
- Szczurek et al. (2017) ) “Imaging chromatin nanostructure with binding-activated localization microscopy based on DNA structure fluctuations”
- Burr et al. (2016) “Mitochondrial Protein Lipoylation and the 2-Oxoglutarate Dehydrogenase Complex Controls HIF1a Stability in Aerobic Conditions”
- Leonard et al. (2016) “SF2312 is a natural phosphonate inhibitor of enolase”
- Roche et al. (2016) “Identification of non-coding genetic variants in samples from hypoxemic respiratory disease patients that affect the transcriptional response to hypoxia”
- Taylor et al. (2016) “Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility”
- Timpano and Uniacke (2016) “Human Cells Cultured Under Physiological Oxygen Utilize Two Cap-binding Proteins to Recruit Distinct mRNAs for Translation”
- Zhao et al. (2016) “Proximal environment controlling the reactivity between inorganic sulfide and heme-peptide model”
From: Burr et al. (2016) “Mitochondrial Protein Lipoylation and the 2-Oxoglutarate Dehydrogenase Complex Controls HIF1a Stability in Aerobic Conditions” Cell Metabolism 25, 740–752
To find out more about Hypoxystations or other DWS products, please call +44 (0) 1274 595728 or email sales@dwscientific.co.uk
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