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Whitley Microaerobic Workstations from Don Whitley Scientific are designed to allow users to control oxygen, carbon dioxide and hydrogen within safe working limits.
By using sophisticated sensors, actual gas levels can be monitored on the touchscreen, giving the user confidence that their samples are being incubated for the appropriate atmosphere.
M series workstations create accurate and reproducible conditions within safe operating parameters. They provide the most flexibility of any models in the Don Whitley Scientific range as they allow Anoxic/Anaerobic, Microaerobic or Hypoxic atmospheres to be created if desired.
Microbiological Applications:
• Anaerobic atmosphere: An oxygen free atmosphere.
• Microaerobic atmosphere: An atmosphere suitable for growing organisms that require oxygen levels lower than those found in air. Hydrogen can be included in the atmosphere if required.
Cell/Tissue Culture Applications:
• Hypoxic atmosphere: An atmosphere suitable for growing cells under physiologically relevant conditions, containing oxygen levels of between 0.1% and 20%, along with carbon dioxide at the required concentration (up to 15%).
Microaerobic operation using an M series workstation
For standard operation of Whitley Microaerobic Workstations, four separate gas cylinders of carbon dioxide, oxygen free nitrogen, compressed air and a 10% hydrogen/90% nitrogen mix, need to be connected to the workstation. This allows the user to select a safe workstation atmosphere from the following available ranges:
Oxygen 0 – 20%
Carbon Dioxide 0 – 15%
Hydrogen 0 – 5%
Nitrogen Balance
Hypoxic operation using an M series workstation
For hypoxic operation only carbon dioxide, oxygen free nitrogen and compressed air should be connected to the workstation. As hydrogen is not required no gas needs to be connected to the 10% hydrogen/90% nitrogen gas input. This will allow the user to choose from the following:
Oxygen 0 – 20%
Carbon Dioxide 0 – 15%
Nitrogen Balance
Anoxic/anaerobic operation using an M series workstation
The M Series can be operated anaerobically (Anoxic conditions) by introducing a catalyst. Any oxygen then present in the workstation atmosphere combines with hydrogen to create water. The catalyst facilitates this reaction and any excess water condenses on a chilled plate and is automatically removed from the workstation.
M35 Microaerobic Workstation used in Campylobacter jejuni research
For more information – email us at sales@dwscientific.co.uk or call us on +44 (0) 1274 595728
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